Jun 13, 2013· Newly emerged flies were not sexed. Males and females were enclosed together in a cotton mesh cage (40 cm W × 25 cm H × 25 cm D) at 24 ± 2 °C with 40–50% relative humidity. Flies were fed with 10% sugar water ad libitum and, once a day, with citrated bovine blood. Experiments were conducted with 2–4-day-old flies.
