Shui Qing Ye, in Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas, 2002. 4. Cloning, Sequencing, and Analyzing of SAGE Tags. Ligation of SAGE ditags to form concatemers. Purified ditags are ligated to concatemers for 3 hr at 16°C in a 10-μl reaction with 1 μl T4 DNA ligase (5 U/μl, . No. 15224-041, GIBCOBRL), 2-μl 5 × ligase buffer, 7 μl SAGE ditag samples.
